인간 골막기원세포와 Polydioxanone/Pluronic F127 담체를 이용한 골형성
In vivo Osteogenesis of Cultured Human Periosteal-derived Cells and Polydioxanone/Pluronic F127 Scaffold
박봉욱, 변준호, 이진호, 오세행, 김상준, 하영술, 전명화, 맹근호, 노규진, 김종렬,
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박봉욱 ( Park Bong-Wook ) - 경상대학교 의학전문대학원 구강악안면외과학교실
변준호 ( Byun June-Ho ) - 경상대학교 의학전문대학원 구강악안면외과학교실
이진호 ( Lee Jin-Ho ) - 한남대학교 생명나노과학대학 신소재공학과
오세행 ( Oh Se-heang ) - 한남대학교 생명나노과학대학 신소재공학과
김상준 ( Kim Sang-June ) - 한남대학교 생명나노과학대학 신소재공학과
하영술 ( Hah Young-Sool ) - 경상대학교병원 임상의학연구소
전명화 ( Jeon Myoung-Hwa ) - 경상대학교 수의과대학 수의산과
맹근호 ( Maeng Geun-Ho ) - 경상대학교 수의과대학 수의산과
노규진 ( Rho Gyu-Jin ) - 경상대학교 수의과대학 수의산과
김종렬 ( Kim Jong-Ryoul ) - 온 종합병원 턱얼굴센터
KMID : 0360120120340060384
Abstract
Purpose: The purpose of this study is to examine in vivo osteogenesis of cultured human periosteal-derived cells and polydioxanone/pluronic F127 scaffold.
Methods: Two one-year-old miniature pigs were used in this study. $2{\times}10^6$ 수식 이미지 periosteal-derived cells in 1 mL medium were seeded by dropping the cell suspension into the polydioxanone/pluronic F127 scaffold. These cell-scaffold constructs were cultured in osteogenic Dulbecco’s modified Eagle’s medium for 7 days. Under general anesthesia with azaperone and tiletamine-zolazepam, the mandibular body and ramus of the pigs were exposed. Three bony defects were created. Polydioxanone/pluronic F127 scaffold with periosteal-derived cells and the scaffold only were implanted into each defect. Another defect was left empty. Twelve weeks after implantation, the animals were sacrificed.
Results: New bone formation was clearly observed in the polydioxanone/pluronic F127 scaffold with periosteal-derived cells. Newly generated bone was also observed in the scaffold without periosteal-derived osteoblasts and empty defect, but was mostly limited to the periphery.
Conclusion: These results suggest that cultured human periosteal-derived cells have good osteogenic capacity in a polydioxanone/pluronic F127 scaffold, which provides a proper environment for the osteoblastic differentiation of these cells.
키워드
Periosteal-derived cells;Polydioxanone scaffold;In vivo osteogenesis
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